Dear Colleague,
Welcome to part one of an eight part email series in which we will share resources and advice on 3D microscopy of large samples. We will begin with discussing physical vs. optical sectioning for the generation of 3D images.
Physical sectioning of samples for microscopy facilitates a range of options for imaging 3D samples. But it damages the tissue structures, causing issues with anatomical alignment when re-constructing the 3D tissue post acquisition, and is extremely time consuming.
Optical sectioning, imaging the entire 3D volume at once, is a superior method but has the drawback in limited penetration due to light scattering or absorption. However, tissue clearing offers a practical solution to this drawback as it renders the tissue transparent, minimizing light scattering and light absorption and allowing deep imaging of tissues, organs or even whole-organisms.
To understand the science behind the need for tissue clearing, discover what tissue clearing can achieve and see an overview of three different types of tissue clearing methods, you can watch our Fundamentals of tissue clearing webinar.
Look out for our next email, where we will dive deeper into the different tissue clearing methods available and compare organic and aqueous tissue clearing methods.
Kind regards,
Byron Hartman
Microscopy Product Manager at Miltenyi Biotec
Want to learn more about light sheet microscopy?
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