Dear Colleague,
In our last email, we discussed the need for tissue clearing for 3D imaging of large samples in order to minimize light scattering and absorption. The variety of tissue clearing methods available means that whether you want to perform immunofluorescence or visualize endogenous fluorescent proteins, there is a clearing technique that is right for you.
Tissue clearing methods can be organic or aqueous in nature and choosing the right method for your research depends on factors including your intended application, storage considerations and toxicity. To help you more fully understand approaches and options for organic and aqueous tissue clearing methods we have two webinars for you:
This organic clearing methods webinar outlines iDISCO, FluoClearBABB and the non-toxic ethyl cinnamate organic tissue clearing techniques.
And this webinar on aqueous tissue clearing methods will help you learn about SeeDB, SHIELD/SWITCH, and PEGASOS.
Keep a look out for our next email, where we will share with you how combining light-sheet microscopy with tissue clearing to image whole mice can provide detailed insight into cancer metastases.
Kind regards,
Byron Hartman
Microscopy Product Manager at Miltenyi Biotec
Watch the the New Frontiers webinar series on demand: